Affinity constant in radioimmunoassay.

M A Ferra, C N Leitão

Abstract


Values for the affinity constant were determined, in three kits, for seric gastrin, following the Scatchard plot and Michaelis-Menten representation. The determination of the affinity constant, K, is of great value for it defines the antigen-antibody complex stability and it gives information about some aspects of the analysis system: sensitivity and antibody saturation.

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